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KMID : 1041219860280010015
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Journal of Sericultural and Entomological Science
1986 Volume.28 No. 1 p.15 ~ p.23
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Comparative Studies of Digestive Fluid Protein of Silkworm, Bombyx mori, Larvae reared on Mulberry Leaves and Artificial Diets
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Abstract
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This study has been carried out to acquire some basic informations on the digestive fluid of Silkworm, Bombyx mori for developing artificial diets.
Silkworms reared on mulberry leaves and artifcial diet, were used in this experiments.
The results obtained are as follows:
1. The Red Fluorescent protein was precipitated in 50% acetone solution and did not dissolved inn-butanol solution, but dissolved in methanol solution.
2. Electrophoretic analysis results of mulberry leaves rearing B. mori and artificial diet rearing B. mori, which has been treated with 50% acctone solution were as follows.
i) There was distinct difference at the position of high mobility.
ii) Red Fluorescence was observed only at the position of first band of mulberry leaves rearing B. mori.
iii) No difference was shown in the electrophoretic patterns of mulberry leaves rearing B. mori on 5th instar 1st~3rd day, but some difference on 5th instar 4th, 5th day.
3. The RFP is the basic protein which has Pl 8~9 according to the isoelectric electrophoresis.
4. The SDS-polyacrylamide gel electrophoresis analysis showed that the molecula weight of RFP was 27,000.
5. The Sephadex G-75 chromatographic analysis showed that there was three peaks between number 16 and 28 in the mulberry leaves rearing B. mori chromatogram, but two peaks between number 18 and 31 in the artifical diet rearing B. mori chromatogram.
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